Asian Journal of Research in Chemistry

Sangita W. Chapke ¹, Madhuri D. Game ¹

Affiliations
1 Vidyabharati College of Pharmacy, C.K. Naidu Road, Camp, Amravati-444602(M.S.), IN

Abstract

A simple, sensitive, accurate and precise UV ‐ spectrophotometric isobestic point method for simultaneous estimation of flunarizine dihydrochloride and propranolol HCl in capsule dosage form has been developed and validated for accuracy, precision, ruggedness, linearity and range. The wavelengths selected for estimation of drugs were 262.5 nm (isobestic point at which both drug exhibit equal absorbance) and 288.8 nm (&#955; <SUB>max</SUB> of propranolol hydrochloride). Linearity for detector response for flunarizine dihydrochloride and propranolol HCL were 1-25 &#181;g/ml and 4-100 μg/ml respectively. The method gives results for high accuracy and high recovery of 99.76±0.08 and 99.38±0.22 for flunarizine dihydrochloride and propranolol HCL respectively. % R.S.D. Values for marketed formulation analysis were found to be less than 2 which indicated good precision and reproducibility of the method. The method was found to be simple, sensitive, accurate and precise.

Keywords

Flunarizine Dihydrochloride, Propranolol HCL, Isobestic Point Method

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Sangita W. Chapke ¹, Madhuri D. Game ¹

Affiliations
1 Vidyabharati College of Pharmacy, C.K. Naidu Road, Camp, Amravati-444602(M.S.), IN

Abstract

A new, simple, sensitive, accurate and precise UV ‐ spectrophotometric absorption correction method for simultaneous estimation of flunarizine dihydrochloride and propranolol HCl in capsule dosage form has been developed and validated for accuracy, precision, ruggedness, linearity and range. The wavelengths selected for estimation of drugs were 253.2 nm for flunarizine dihydrochloride and 288.8 nm for propranolol HCl. Linearity for detector response for flunarizine dihydrochloride and propranolol HCl were 1-25 &#181;g/ml and 4-100 μg/ml respectively. The method gives results for high accuracy and high recovery of 99.15±1.37 and 100.02±0.89 for flunarizine dihydrochloride and propranolol HCl respectively. % R.S.D. Values for marketed formulation analysis were found to be less than 2 which indicated good precision and reproducibility of the method.

Keywords

Flunarizine dihydrochloride, Propranolol HCl, Absorption correction method

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Madhuri D. Game ¹, Koshish B. Gabhane ¹, Dinesh M. Sakarkar ²

Affiliations
1 Vidyabharati College of Pharmacy, C.K. Naidu Road, Camp, Amravati – 444602, (M. S.), IN
2 Sudhakarrao Naik Institute of Pharmacy, Pusad – 445204 (M. S.), IN

Abstract

A simple, sensitive, rapid, accurate and precise UV-spectrophotometric (absorbance correction) method for simultaneous estimation of glimepiride and pioglitazone HCl in pharmaceutical dosage form was developed and validated for accuracy, precision, ruggedness, linearity and range. The wavelengths selected for estimation of drugs were 270.0 nm for pioglitazone HCl and 230.0 nm for glimepiride. Linearity for detector response for glimepiride and pioglitazone HCl were in the range of 2-50 μg/ml and 10-170 μg/ml respectively. The method gives results of high accuracy and high recovery of 100.34 ± 0.25 and 99.54 ± 0.47 for glimepiride and pioglitazone HCl respectively. % R.S.D. values for the marketed formulation analysis were found to be less than 2 which indicated good precision and reproducibility of the method. Commercial capsule formulation was successfully analyzed using the developed method.

Keywords

Glimepiride, Pioglitazone, UV Spectrophotometry, Absorbance Correction Method, Method Validation.

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